Study on the gene expressions in murine intestinal cells before and after immunization with attenuated living vaccine of Salmonella typhi Ty21a by cDNA microarray

The expressions of genes in murine intestinal cells (including epithelial cells and intraepithelial lymphocytes) before and after immunization with Ty21a vaccine were studied by using the cDNA microarray. DNA microarrays were made by spotting PCR products of 490 Ty21a-associated cDNA of mice selected by SSH into a specially treated glass slide. The probe used was prepared by labeling intestinal mRNA of mice before and after immunization with Cy-3dUTP and Cy5-dUTP by means of reverse transcription. The arrays were then hybridized with cDNA probe,and the fluorescent signals scanned.Data thus obtained were analyzed from two repeated experiments.All together,98 differenially expressed genes were scanned out after immunization,in which 92 were up-regulated genes and 6 were down-regulated genes. It suggests that high throughput cDNA microarray is a credible method to profile changes in gene expression,and can be used for the quantitative studies of large scale of gene expression.