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Application of cDNA microarray in the mechanism analysis of drug

Zou Mingjin

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Abstract

Objective:To explore the application of cDNA microarray in the mechanism analysis of drug.Methods:20 rats bearing NuTu-19 cells were randomly divded into two groups,the drug group (n=10) were treated with basil polysaccharide for 50d,while the control group (n=10) treated with physiological saline at same dose,changes of the biology phenotype of tumor cell were observed.Total RNA of the tumor tissue was extracted,and the fluorescent cDNA probes were prepared through reverse transcription of the isolated mRNAs.The RNA samples were labeled with Cy3-dUTP or Cy5-dUTP,then hybridized with the microarrays.Results:Compared with the control group,168 genes in the drug group showed different expression.Conclusion:cDNA microarray technology may be power tools to discover the possible mechanism of drug in gene level,and can provide new informations to accelerate the development of new drugs.

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What this paper is about

Objective:To explore the application of cDNA microarray in the mechanism analysis of drug.Methods:20 rats bearing NuTu-19 cells were randomly divded into two groups,the drug group (n=10) were treated with basil polysaccharide for 50d,while the control group (n=10) treated with physiological saline at same dose,changes of the biology phenotype of tumor cell were observed.Total RNA of the tumor tissue was extracted,and the fluorescent cDNA probes were prepared through reverse transcription of the isolated mRNAs.The RNA samples were labeled with Cy3-dUTP or Cy5-dUTP,then hybridized with the microarrays.Results:Compared with the control group,168 genes in the drug group showed different expression.Conclusion:cDNA microarray technology may be power tools to discover the possible mechanism of drug in gene level,and can provide new informations to accelerate the development of new drugs.

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Available abstract

Objective:To explore the application of cDNA microarray in the mechanism analysis of drug.Methods:20 rats bearing NuTu-19 cells were randomly divded into two groups,the drug group (n=10) were treated with basil polysaccharide for 50d,while the control group (n=10) treated with physiological saline at same dose,changes of the biology phenotype of tumor cell were observed.Total RNA of the tumor tissue was extracted,and the fluorescent cDNA probes were prepared through reverse transcription of the isolated mRNAs.The RNA samples were labeled with Cy3-dUTP or Cy5-dUTP,then hybridized with the microarrays.Results:Compared with the control group,168 genes in the drug group showed different expression.Conclusion:cDNA microarray technology may be power tools to discover the possible mechanism of drug in gene level,and can provide new informations to accelerate the development of new drugs.

Key concepts: Complementary DNA, Microarray, DNA microarray, Reverse transcriptase, Biology, Molecular biology, Microarray analysis techniques, RNA

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Application of cDNA microarray in the mechanism analysis of drug — Research Paper | ScholarLens