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2011•Zhongguo yancao kexueRequires access

Application of Suppression Subtractive Hybridization(SSH) on Tobacco Research

Liming Lu

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Abstract

A new method,termed suppression subtractive hybridization(SSH),has been developed based primarily on the technique suppression PCR and subtractive hybrid.It has many advantages such as high efficiency,high specificity and high sensitivity,and is widely applied in the botany research.This article mainly focuses on the principles,protocol,main advantages and disadvantages,and its application in tobacco research.

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What this paper is about

A new method,termed suppression subtractive hybridization(SSH),has been developed based primarily on the technique suppression PCR and subtractive hybrid.It has many advantages such as high efficiency,high specificity and high sensitivity,and is widely applied in the botany research.This article mainly focuses on the principles,protocol,main advantages and disadvantages,and its application in tobacco research.

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Available abstract

A new method,termed suppression subtractive hybridization(SSH),has been developed based primarily on the technique suppression PCR and subtractive hybrid.It has many advantages such as high efficiency,high specificity and high sensitivity,and is widely applied in the botany research.This article mainly focuses on the principles,protocol,main advantages and disadvantages,and its application in tobacco research.

Key concepts: Suppression subtractive hybridization, Subtractive color, Computational biology, Biology, Sensitivity (control systems), Computer science, Biochemical engineering, Genetics

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