Experiment promotion nerons regeneration and expression of GAP-43 proteins and IGF-1 proteins after focal cerebral ischemia reperfusion injury in rats

Objective To explore of the the mechanism of the expression of GAP-43 proteins inhibition neurons apoptosis and IGF-1 proteins promotion neurons regeneration after cerebral ischemia reperfusion injury in Wistar rats.Methods The healthy adult of male Wister rats (80) were divided into GAP-43 group and IGF-1 group,per group (40),and they divided andomly into normal control group and sham-operation group and in ischemic 1h reperfusion 2h,6h,12h,24h,48h,3d,7d,14d group(n=4).The models with cerebral ischemic reperfusion were induced by intraluminal middle cerebral artery occlusion (MCAO) with a nylon monofilament suture.And using immunohistochemistry technique detecte the condition of the expression of GAP-43 proteins and IGF-I proteins in apoptosis of neurons,and by a computer image analysis system.Results GAP-43 group:Showing the expression of background of GAP-43 of neurons in hippocampus and cortical area and striatal area in cerebral ischemia reperfusion in 2h,The expression of GAP-43 showed gradually increase in 6h、12h、24h、48h,the expression of it reached peak in 7d,the expression of it reached lowest the level in 14d,P005.It compare with sham-operation is significancely difference,P0.05.IGF-1 group:Showing the expression of background of positive GAP-43 of budding cells in hippocampus and cortical area and striatal area on normal control group and sham-operation group.The expression of IGF-1 is clearly increase in ischemic reperfusion in 2h,and reached peak in 24h,P0.05,and expressed constant in 48h.It still keeped high the expression from 3d to 14d,P0.05.Conclusion The expression of GAP-43 and IGF-1 participate in the inhibition and promotion of neuron axon regeneration.