The expression and significance of growth associated protein-43 after cerebral ischemic reperfusion in rats

Objective To explore the expression of growth associated protein-43(GAP-43)to the change of plasticity of neurons axon regeneration after cerebral ischemic reperfusion injury in rats.Methods The healthy adult male Wister rats (40) were divided randomly into normal control group sham-operation group and ischemic 1h reperfusion 2h,6h,12h,24h,48h,3d,7d,14d groups(n=4).The models with cerebral ischemic reperfusion were induced by intraluminal middle cerebral artery occlusion (MCAO) with a nylon monofilament suture.And using immunohistochemistry technique detect the expression of GAP-43 protein and investigating alteration of neurons axon regeneration by a computer image analysis system.Results Background expression of GAP-43 in reperfusion 2h was detected in hippocampus and cortex and striatum,while the expression of GAP-43 declined increased in 6h,12h,24h,48h.The expression peak appeared on 7d,the lowest expression appeared on 14d.The expression of the ischemic reperfusion group was higher than sham-operation group,while normal control group wasn't showed it.The lesion area appeared the budding sign of neurons axon in cerebral ischemic reperfusion from 48h to 7d,and showed synaptic fiber.Conclusion GAP-43 has nonspecific reaction and promote the repair of neurons and regeneration after cerebral ischemic reperfusion injury in rats.