Correlation between quantified HPLC fingerprint and its anti-oxidation activity of Salviae miltiorrhizae Radix Et Rhizoma

Objective To establish the 280 nm quantitative HPLC fingerprints of Salviae miltiorrhizae Radix Et Rhizoma and determine its antioxidant activity,between which the bivariate correlation analysis was performed.Methods The fingerprints of Salviae miltiorrhizae Radix Et Rhizoma were established by HPLC method and evaluated by the systematically quantified fingerprint method(SQFM).Meanwhile the cleaning ratio(CLR) of hydroxyl free radical were also determined and the bivariate correlation analysis was employed to investigate the conjunction between each fingerprint and the CLR calculated by SPSS 16.0 software.Results S9 was the most antioxidant activity within the 10 batches of samples,and the correlation analysis showed that 18 fingerprint peaks(2,3,9,10,11,14,16,18,19,21,23,24,29,31,32,34,35,and 43) were positively correlated with the anti-oxidation activity significantly among the 44 common chromatographic peaks in the 280 nm HPLC fingerprints.Conclusion The antioxidant profile relationship between the HPLC fingerprint peaks and the CLR has been established,which may serve as a reference for screening the antioxidant active ingredients to better control the quality of Salviae miltiorrhizae Radix Et Rhizoma.