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Studie on the fingerprint of Radix et Rhizoma Salviae Miltiorrhizae

LI Qiong-hong

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Abstract

Objective To establish the method of fingerprint analysis of Radix et Rhizoma Salviae Miltiorrhizae by HPLC.Methods Kromasil C_(18)((4.6 mm)×(250 mm),(5 μm))column was used.The mobile phase was composed of 0.02% H_3PO_4-CH_3CN and 0.02% H_3PO_4-H_2O with a linear gradient elution.The flow rate was(1.0 mL)·min~(-1) with DAD detected at(280 nm).The column temperature was maintained at(40 ℃).Results The 14 common peaks were pinpointed by the determination of the 11 Radix et Rhizoma Salviae Miltiorrhizae samples of different specifications gathered from different places.The similarities,which were proved to be higher than 0.9,were calculated with the help of The Similarity Calculation Soft of Fingerprint Chromatography of Traditional Chinese Medicine.Conclusions This method is accurate,reliable and provides a scientific basis for controlling the quality of Radix et Rhizoma Salviae Miltiorrhizae.

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What this paper is about

Objective To establish the method of fingerprint analysis of Radix et Rhizoma Salviae Miltiorrhizae by HPLC.Methods Kromasil C_(18)((4.6 mm)×(250 mm),(5 μm))column was used.The mobile phase was composed of 0.02% H_3PO_4-CH_3CN and 0.02% H_3PO_4-H_2O with a linear gradient elution.The flow rate was(1.0 mL)·min~(-1) with DAD detected at(280 nm).The column temperature was maintained at(40 ℃).Results The 14 common peaks were pinpointed by the determination of the 11 Radix et Rhizoma Salviae Miltiorrhizae samples of different specifications gathered from different places.The similarities,which were proved to be higher than 0.9,were calculated with the help of The Similarity Calculation Soft of Fingerprint Chromatography of Traditional Chinese Medicine.Conclusions This method is accurate,reliable and provides a scientific basis for controlling the quality of Radix et Rhizoma Salviae Miltiorrhizae.

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Available abstract

Objective To establish the method of fingerprint analysis of Radix et Rhizoma Salviae Miltiorrhizae by HPLC.Methods Kromasil C_(18)((4.6 mm)×(250 mm),(5 μm))column was used.The mobile phase was composed of 0.02% H_3PO_4-CH_3CN and 0.02% H_3PO_4-H_2O with a linear gradient elution.The flow rate was(1.0 mL)·min~(-1) with DAD detected at(280 nm).The column temperature was maintained at(40 ℃).Results The 14 common peaks were pinpointed by the determination of the 11 Radix et Rhizoma Salviae Miltiorrhizae samples of different specifications gathered from different places.The similarities,which were proved to be higher than 0.9,were calculated with the help of The Similarity Calculation Soft of Fingerprint Chromatography of Traditional Chinese Medicine.Conclusions This method is accurate,reliable and provides a scientific basis for controlling the quality of Radix et Rhizoma Salviae Miltiorrhizae.

Key concepts: Radix (gastropod), Chromatography, Fingerprint (computing), Chemistry, Gradient elution, High-performance liquid chromatography, Traditional medicine, Computer science

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