Western blot analysis on the soluble greasy and degrease adult worm antigens of Clonorchis sinensis

Objective To find out the valuable specific antigens for immunodiagnosis of clonorchiasis.Methods The greasy,degrease adult worm antigens of Clonorchis sinensis were separated by SDS-PAGE and transferred on the nitro-cellulose membrane,then were recognized by the patient's sera of clonorchiasis,schistosomiasis and paragonimiasis respectively.Results 14 protein bands were recognized by the sera of clonorchiasis in the greasy antigens of adult worms,among which the protein bands with relative molecular weight(MW) of 180,170,100,67,41,37,35,32,26 ku were main bands,in addition,the bands of 180,170,37,35 ku could also cross-react with sera of schistosomiasis and paragonimiasis.There were 10 proteins bands recognized by sera of clonorchiasis in degrease adult worm antigens of C.sinensis,among which the protein bands with MW of 180,100,67,37,35,24,20 ku were main bands,in addition,the protein bands of 180 ku could be recognized by the sera of schistosomiasis and paragonimiasis,the band of 78 ku could also be recognized by the sera of schisotosomiasis.Conclusion The degreasing for C.sinensis adult worm antigen could reduce the numbers of the fraction antigens which could cross-react with sera of schistosomiasis and paragonimiasis. The fraction of 100,67,41,32,26 ku in the greasy antigen and 100,67,37,35,24,20 ku in the degreased antigens of C.sinensis adult worms are the main specific antigens.It could be applied to the specific immunodiagnosis of clonorchiasis.