Effects of temperature and storage time on the pig sperm acrosin activity.

The purpose of this study was to explore the temperature and storage time on the pig sperm acrosin activity,which was detected by UV.The results showed that:(1)Fresh,cold shock and heat shock sperm acrosin activities were 5.38,5.20 and 5.16 mIU per 106 sperm,respectively,but the difference was not significant.(2)Fresh sperm acrosin activities at 4℃,17℃,25℃,30℃,37℃ and 39℃ were 4.29,5.01,5.03,5.17,4.78 and 4.61 mIU per 106 sperm,respectively.Sperm acrosin activity increase along with temperature increasing.When the temperature reached 30℃,acrosin activity reached the highest,the difference was significant between 4℃ and 30℃(P0.05).(3)When the preservation of more than 1 d at 4℃,the sperm acrosin activity decreased significantly(P0.05);when the preservation of more than 2 d at 17℃,25℃ and 39℃,acrosin activity decreased significantly(P0.05).The efficiency of preservation at 17℃ was best.In conclusion,the temperature affects the pig sperm acrosin activity,and the temperature of liquid semen Preservation was suitable for 17℃.Under this temperature the pig sperm can be saved for 3 d.