Preparation and immune effect of inactivated porcine reproductive and respiratory syndrome vaccine.

Objective To prepare an inactivated oil emulsion vaccine with a porcine reproductive and respiratory syndrome virus (PRRSV)Anhui strain. Methods PRRSV was subcultured and proliferated in Marc-145 cells, and prepared into inactivated oil emulsion vaccine which was subjected to examination on physical property as well as sterility and safety tests. The qualified vaccine was used for immunization of healthy piglets by intramuscular injection, and the serum antibody level and protection were determined. The immune effects of vaccine by various schedules, as well as those of the prepared vaccine and commercial inactivated vaccines, were compared. Results The optimal dosage of prepared vaccine for each piglet was 2 ml. The serum antibody level of immunized piglets reached a peak value 14 d after immunization. The protective rate of the prepared vaccine against challenge with the same virus strain was 80%(4 / 5). Both immunizations with two doses of inactivated vaccine and with one dose of inactivated vaccine and one dose of live attenuated vaccine showed good immune effects. The immune effect of prepared inactivated vaccine showed no significant difference with that of commercial inactivated vaccine No. 1, while superior to that of No. 2. Conclusion Inactivated PRRSV oil emulsion vaccine was successfully prepared, which was safe, effective, showed good immune effect and was worthy of popularization.