Development and Efficacy of Concentrated Inactivated Vaccines against Highly Pathogenic Porcine Reproductive and Respiratory Syndrome

[Objective] The research aimed to develop vaccine against highly pathogenic porcine reproductive and respiratory syndrome.[Method]Using virus and concentrated virus as antigen,and using formaldehyde and β-propiolactone as inactivator,four inactivated vaccines against highly pathogenic porcine reproductive and respiratory syndrome were prepared respectively. After the physical behavior,steriling test,and the safety test,the immunity effect of these four kinds of vaccines and one commercial vaccine were compared. [Result]These four kinds of vaccine all had good safety. After immunization 5 weeks,the specific antibody titers produced by concentrated inactivated vaccines B and D were 80% and 100% respectively,while no specific antibody titers produced by the unconcentrated inactivated vaccines A,C and the commercial vaccine E. [Conclusion] The immune effect of inactivated vaccines prepared with concentrated virus was better than those prepared with unconcentrated virus and the commercial vaccine in this study.