Tissue Culture and Plantlet Regeneration of Polygonaturm sibiricum

The technique of plantlet regeneration and callus induction was researched by different explants from Polygonaturm sibiricum in vitro.The results showed that callus could be induced from the excised rhizome,young stem and leaves cultured on the medium with MS+6-BA 2.0 mg·L-1+2,4-D 1.0 mg·L-1 and MS+6-BA 2.0 mg·L-1+NAA 1.0 mg·L-1.The embryonic callus could be multiplied better on the medium supplemented with MS+6-BA 2.0 mg·L-1+2,4-D 2.0 mg·L-1and MS+ 6-BA 2.0 mg·L-1+NAA 2.0 mg·L-1.The adventitious bud could be regenerated from callus on the medium MS+6-BA 3.0 mg·L-1+IAA 1.0 mg·L-1,and regeneration frequency was 66.33%,the number of buds per callus was up to 5.2 in average.Rooting occurred on the MS medium addition of 0.5 mg·L-1 IAA,and the rooting rate could reach up to 86.67%.