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2013•Anhui Nongye Daxue xuebaoRequires access

Tissue culture and rapid propagation of Carpinus tientaiensis

Zhe Chen

Open publisher page 1 citations

Abstract

To establish a system of tissue culture and plantlet regeneration from Carpinus tientaiensis, stem segments, buds, or buds under germination and leaves were used as explants. The results showed that the optimum medium for induction and multiplication of adventitious shoots was 1/2 MS+6-BA 2.0 mg·L-1+NAA 0.2 mg·L-1+ PVP 0.2% when buds was used as explants. The medium for cultivating strong seedling was 1/2 MS+6-BA 0.5 mg·L-1+ NAA 0.05 mg·L-1, and the optimum medium for root regeneration was 1/4 MS+IBA 1.0 mg·L-1+NAA 0.1 mg·L-1.

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What this paper is about

To establish a system of tissue culture and plantlet regeneration from Carpinus tientaiensis, stem segments, buds, or buds under germination and leaves were used as explants. The results showed that the optimum medium for induction and multiplication of adventitious shoots was 1/2 MS+6-BA 2.0 mg·L-1+NAA 0.2 mg·L-1+ PVP 0.2% when buds was used as explants. The medium for cultivating strong seedling was 1/2 MS+6-BA 0.5 mg·L-1+ NAA 0.05 mg·L-1, and the optimum medium for root regeneration was 1/4 MS+IBA 1.0 mg·L-1+NAA 0.1 mg·L-1.

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Available abstract

To establish a system of tissue culture and plantlet regeneration from Carpinus tientaiensis, stem segments, buds, or buds under germination and leaves were used as explants. The results showed that the optimum medium for induction and multiplication of adventitious shoots was 1/2 MS+6-BA 2.0 mg·L-1+NAA 0.2 mg·L-1+ PVP 0.2% when buds was used as explants. The medium for cultivating strong seedling was 1/2 MS+6-BA 0.5 mg·L-1+ NAA 0.05 mg·L-1, and the optimum medium for root regeneration was 1/4 MS+IBA 1.0 mg·L-1+NAA 0.1 mg·L-1.

Key concepts: Plantlet, Explant culture, Shoot, Tissue culture, Germination, Botany, Seedling, Regeneration (biology)

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