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2010•Zhongguo zhongyiyao xinxi zazhiRequires access

Study on quality standard of Shengfa Liniment.

Cai Lv-lv, Pan Jin-huo, Guojun Yan

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Abstract

Objective To establish the method for quality standard of Shengfa Liniment.Methods Herba Ecliptae,Folium Mori and Cortex Phellodendron were identified by TLC.HPLC method for determining berberine in Shengfa Liniment was established.The column was Agilent C-18(5 μm,4.6 mm×250 mm),the mobile phase was acetonitrile-0.033 mL/L potassium dihydrogen phosphate(35:65).The flow rate was 1.0 mL/min.The detection wavelength was at 424 nm.Results The spots on the TLC plate were clear.The negative control sample had no interference.A good linearity for berberine was obtained in the range of 0.031 36-0.188 16 μg,r =0.999 7.The average recovery was 101.23%,RSD=1.79%(n =6).Conclusion The methods are specific,accurate and reproducible,and can be used for the quality control of the product.

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What this paper is about

Objective To establish the method for quality standard of Shengfa Liniment.Methods Herba Ecliptae,Folium Mori and Cortex Phellodendron were identified by TLC.HPLC method for determining berberine in Shengfa Liniment was established.The column was Agilent C-18(5 μm,4.6 mm×250 mm),the mobile phase was acetonitrile-0.033 mL/L potassium dihydrogen phosphate(35:65).The flow rate was 1.0 mL/min.The detection wavelength was at 424 nm.Results The spots on the TLC plate were clear.The negative control sample had no interference.A good linearity for berberine was obtained in the range of 0.031 36-0.188 16 μg,r =0.999 7.The average recovery was 101.23%,RSD=1.79%(n =6).Conclusion The methods are specific,accurate and reproducible,and can be used for the quality control of the product.

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Available abstract

Objective To establish the method for quality standard of Shengfa Liniment.Methods Herba Ecliptae,Folium Mori and Cortex Phellodendron were identified by TLC.HPLC method for determining berberine in Shengfa Liniment was established.The column was Agilent C-18(5 μm,4.6 mm×250 mm),the mobile phase was acetonitrile-0.033 mL/L potassium dihydrogen phosphate(35:65).The flow rate was 1.0 mL/min.The detection wavelength was at 424 nm.Results The spots on the TLC plate were clear.The negative control sample had no interference.A good linearity for berberine was obtained in the range of 0.031 36-0.188 16 μg,r =0.999 7.The average recovery was 101.23%,RSD=1.79%(n =6).Conclusion The methods are specific,accurate and reproducible,and can be used for the quality control of the product.

Key concepts: Chromatography, Berberine, Quality standard, Folium of Descartes, High-performance liquid chromatography, Chemistry, Potassium, Negative control

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