PNS on pulmonary fibrosis in mice serum collagen IV-C, and the intervention of hyaluronic acid.

Objective:To investigate the effect of PNS on pulmonary fibrosis and the hyaluronic acid in mice serum collagen Ⅳ-C.Methods:C57BL/6 mice 60 were divided into PNS high dose group, PNS medium dose group, low dose group, prednisone group, model group, sham operation group, n=10.The mice were given a single intratracheal instillation of bleomycin hydrochloride except mice of sham operation group, while which were given a single intratracheal instillation with saline.The mice were given medicine 2 days after modeling until before they were killed.PNS for high, medium and low dose group were 120, 60, 30 mg/kg/d, respectively; Prednisone was 0.56mg/kg.Sham operation group, model group were given equal volume of saline.At 28d after injection, the serum levels of collagen Ⅳ-Cand hyaluronic acid content were detected.Results:Ⅳ-C collagen, hyaluronic acid in the lungs of mice in model group were significantly higher than that in sham operation group (P0.01), Compared with model group, PNS high dosages could reductionⅣ-C collagen, hyaluronic acid, which in the role of high dose group decreased more significantly (P0.01); Conclusion:PNS could treat pulmonary fibrosis by reducing pulmonary fibrosis in mice.