2012•Zhonghua zhongyiyao zazhiRequires access
Effect of panax notoginsenosides on collagen metabolism of dimethynitrosamine-induced hepatic fibrosis rats
Yongsheng Zhang, Shan Xu, Yufang Zhao, Zhu Feiye
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Abstract
Objective: To observe the effect of panax notoginsenosides(PNS) on collagen metabolism of hepatic fibrosis model rats.Methods: DMN hepatic fibrosis model was used in this experimental research.After injected DMN,the rats were divided into 4 groups,including,a.PNS Treatment Group;b.INF-γ Treatment Group;c.PNS INF-γ Treatment Group;d.Negative Control Group;In addition,10 rats were arranged to be the Normal Control Group during the whole study.All rats received 8 weeks' treatment by PNS,INF-γ,PNS INF-γ and saline solution,respectively.All rats were killed under anesthesia to observe the effect of PNS on the collagen metabolism of hepatic fibrosis rats compared with INF-γ after 8 weeks' treatment.The contents of liver Hydroxyproline,the index of liver function and Hyaluronic Acid(HA) were used to evaluate the function of PNS on the collagen metabolism on hepatic fibrosis rat model compared with IFN-γ.Histological sections of liver from the killed rats were stained by Hematoxylin and Eosin,Sirius Red Stains,respectively.Results: After 8 weeks' treatment,the indexes of the liver function and HA in the PNS Treatment Group rats were markedly decreased compared with the Negative Control Group rats(P0.05),the contents of Hydroxyproline and the grade of hepatic fibrosis in PNS Treatment Group rats liver were lower than those of Negative Control Group,respectively.Conclusion: PNS can improve the collagen contents on hepatic fibrosis rats by not only reduce the index of liver Hydroxyproline,but also by decrease the grade of hepatic fibrosis.