2010•Zhongguo yiyuan ganranxue zazhiRequires access
Mobile genetic elements in multidrug-resistant Pseudomonas aeruginosa.
Zhang Qing, Wei Ming Li, Wang JinDuo
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Abstract
OBJECTIVE To investigate the distribution of mobile genetic elements:plasmids,insertion sequences,transposons,and integrons in multidrug-resistant Pseudomonas aeruginosa(MDRPA) strains.METHODS From May 2009 to Nov 2009,20 strains of MDRPA isolated from sputum were collected from Shanghai No.5 People′s Hospital affiliated to Fudan University.Then,14 kinds of mobile genetic elements(traA,traF,trbC,SCR1,ISCR3/14,IS1133,ISpa7,ISEcp1,tnpU,tnpA/Tn21,tnsA,intⅠ1,intⅠ2,and intⅠ3) were analyzed by PCR and verified by DNA sequencing.RESULTS Among 20 strains of MDRPA,10 strains(50.0%),12 strains(60.0%),12 strains(60.0%),12 strains(60.0%),and 11 strains(55.0%)were detected to carry trbC,ISCR1,ISpa7,tnpA/Tn21,and intⅠ1,respectively.However,the rest 9 kinds of genes couldn′t be detected.Positive genes could be divided into 3 kinds of positive modes,and the mode(trbC+ISCR1+ISpa7+tnpA/Tn21+intⅠ1) had the highest rate(9 strains,45.0%).CONCLUSION Four kinds of mobile genetic elements including plasmids,insertion sequences,transposons,and integrons are all detected in this group of 20 strains of MDRPA,and acquired resistance genes induced by these mobile genetic elements cause the recipient strains to express multi-drug resistance.It′s the first report to detect 14 kinds of mobile genetic elements in China and it′s the first report that tnpA/Tn21 and Ispa7 are detected in China.