2010•Zhongguo yiyuan ganranxue zazhiRequires access
Mobile genetic elements in multidrug-resistant Acinetobacter baumannii.
Zhu JianMing, MA Zhao-long, Jiang RuJin, WU Kang-le
Open publisher page 0 citations
Abstract
OBJECTIVE To investigate the distribution of mobile genetic elements in multidrug-resistant Acinetobacter baumannii(MDR-ABA) isolated from clinical samples.METHODS Sixty-two strains of MDR-ABA were isolated from inpatients.Then,10 kinds of mobile genetic elements: genetic markers of conjugal plasmids(traA and trbC),insertion sequences(ISaba1,IS1133 and ISEcp1),genetic markers of transposons(merA and tnsA),and genetic markers of integrons(intⅠ1,intⅠ2 and intⅠ3) were analyzed by PCR and verified by DNA sequencing.Combining with genetic markers of transposons(tnpU and tnp513) reported before,all data were analyzed as a whole.RESULTS In 62 strains of MDR-ABA,55 strains(88.7%),34(54.8%),35(56.5%)and 34(54.8%) were detected to carry ISaba1,tnpU,tnp513 and intⅠ1,respectively.However,the rest 8 kinds of genes couldn′t be detected out.Furthermore,55 strains(88.7%)were detected out to carry at least 1 kind of gene.In addition,33 strains(53.2%)were detected to carry 4 kinds of genes together.CONCLUSIONS The group of MDR-ABA shows multidrug resistance to beta-lactams,aminoglycosides,quinolones,tetracycline and trimethoprim-sulfamethoxazole antimicrobial agents might result from high rate of mobile genetic elements,such as ISaba1,tnpU,tnp513 and intⅠ1,harboring on strains.It′s the first report that insertion sequence ISaba1,and genetic markers of transposons tnpU and tnp513 are detected in MDR-ABA in China.