Inhibitory effect of evodiamine on proliferation of human colon cancer lovo cells.

Objective To investigate the in vitro and in vivo inhibitory effects of evodiamine(Evo)on the proliferation of human colon cancer lovo cells as well as the relevant mechanism. Methods The effects of Evo on the proliferations of lovo cells, human breast cancer MDA-MB-231 cells and human lung cancer A549 cells were determined by MTT method. The effects of Evo on the cell cycle and apoptosis of lovo cells were analyzed by flow cytometry. Nude mice bearing lovo cells were treated with Evo, based on which a tumor growth curved was plotted. The effects of Evo on the expressions of Bcl-2 and procaspase-3 in lovo cells and tumor tissue were determined by Western blot. Results Evo inhibited the proliferation of lovo cells (P 0. 01)and promoted that of MDAMB-231 cells(P 0. 01)significantly, while showed no significant effect on A549 cells. Evo arrested lovo cells at S phase(P 0.01), induced the apoptosis of the cells in a time-dependent mode(P 0. 01), and inhibited the growths of tumors in the mice bearing lovo cells (P 0. 05). Western blot showed both in vitro and in vivo inhibitory effects of Evo on expressions of Bcl-2 and procaspase-3. Conclusion Evo may activate caspase-3 and induce cell apoptosis by inhibiting the expression of Bcl-2, thus inhibit the proliferation of lovo cells.