Effects of Oleanolic Acid on Proliferation and Apoptosis of Human Colon Cancer LoVo Cell

Objective To study the effects of oleanolic acid(OA) on proliferation and apoptosis of colon cancer LoVo cell lines in vitro. Methods The human colon cancer LoVo cells were treated with 2.00～32.00 mg·L-1 OA for 12～96 h.The influence of OA on proliferation of LoVo cell in vitro was detected with MTT method.Cell apoptosis rate was determined by flow cytometry,and protein expressions of Bcl-2,Bax,p53 and Caspase-3 were investingated by Western blot analysis.The apoptosis was observed by electron microscopy. Results OA inhibited the proliferation of LoVo cells in a dose and time dependent way.It significantly induced apoptosis of LoVo cells and blocked cells at G2/M phase.The apoptosis rates were 10.32%～14.24%(P0.05) after 48 hours.With the increase of concentrateion of OA,the expressions of Bax,p53 and Caspase-3 were increased,while the level Bcl-2 protein was decreased. Conclusion OA induces apoptosis of colon cancer LoVo cell lines,which may be related to inhibition of Bcl-2 and promotion overexpressing of Bax,p53 and Caspase-3.