Simultaneous determination of chlorogenic acid, cryptochlorogenicacid, caffeic acid, naringin, hesperidin and linarin in Xiao'erjinningoral liquid by an HPLC method

OBJECTIVE: To develop a HPLC method for the simultaneous determination of chlorogenic acid, cryptochlorogenic acid, caffeic acid, naringin, hesperidin and linarin in xiao' erjinning oral liquid. METHOD: The chromatographic separation was achieved on a Lichrospher C18 (4.6 mm x 250 mm, 5 microm) column with a mobile phase which was composed of acetonitrile(A) and 0.4% phosphoric acid(B) for gradient elution (10:90-18:82-27:73). The flow rate was (0.8-1.1-0.8) mL x min(-1), the column temperature was 30 degrees C and the detection wavelength was set at 300 nm. RESULT: The results showed that 6 effective components were separated well and showed good linearity. The average recoveries were between 95%-105%. CONCLUSION: The method is proved to be rapid, accurate, credible and repeatable. It can be used for the quality control of Xiao'erjinning oral liquid.