Boiling sterilization for oral air gun: an experimental study.

OBJECTIVE To evaluate the boiling sterilization effect on the oral air gun.METHODS After the oral air gun artificially polluted with hepatitis B virus and Mycobacterium tuberculosis,and disinfected by boiling method the effect of sterilization was detected by fluorescence quantitative polymerase chain reaction(PCR) technology for hepatitis B virus DNA and M.tuberculosis.RESULTS After Boiling sterilization for 10min,the hepatitis B virus and M.tuberculosis were not detected.The sterilization passing rate reached 100 percent there was a statistically significant difference(P0.01),compared with the sterilization for 2 or 5min.Between sterilization for 10min,and 15min for the hepatitis B virus and M.tuberculosis there was no significant difference in sterilizing effect.After boiling sterilization for 10min,the positive rate of HBV DNA and M.tuberculosis in saline or serum was 0.CONCLUSION The boiling sterilization can reach the same effect as 2% glutaraldehyde disinfectant immersion and autoclave sterilization,and its priority is simple but effective.