Effect of autoclave on sterilization of dental instruments:A experimental study.

Objective:To evaluate the sterilizing effect of autoclave on the common medical instruments of oral consulting rooms.Methods:20 dental handpieces and 20 tooth cleaning handpieces were chosen randomly,and sterilixed with Tuttnauer-2540MK autoclave.After that,the bacterial sampling was performed from these sterilized handpieces,and the bacterium wa counted after 48 hours.with the ELISA,the instruments handled by blood serum of HBsAg positivity were detected after sterilization.The standard was S/N ≥2.1 as positive,or negative.Results:The effect of Tuttnauer-2540 MK autoclave on sterilization of bacteria and HBsAg was excellent.On 15 minutes after sterilization,the killing rate of bacterium of 9 samples in dental handpiece group was 100% and one was 99.84%,the killing rate of bacterium of 8 samples in tooth cleaning handipec group was 100% and one was 99.89%.After sterilization,the S/N values of 20 instruments contaminated by the blood serum of HBsAg pasitivety were all less than 2.1,and all were negative.Conclusion:The sterilization of Tuttnauer-2540MK autoclave costs a short time It can make the complicated dental instruments to achieve the requisition of sterilization and HBsAg deactivation.