The Improvement of Extraction Method for High-quality Genomic DNA from Processing Tomato

The processing tomato will accumulate large quantities of polysaccharides,protein and other secondary metabolites during it grow process,and it is difficult to extract high-quality DNA or the extracted DNA can be broken down easily with the reported DNA extraction method.In this paper an improved CTAB method was used to extract genomic DNA from young leaves and old leaves of processing tomato.The extracted DNA was detected by UV spectrophotometer and gel electrophoresis,the results showed that the extracted DNA had high purity and good integrity.The PCR amplification can get obvious polymorphic fragments of extracted DNA,so it can be directly used for subsequent experiments.