RP-HPLC Determination of Amlodipine Besylate and Atorvastatin in Amlodipine Besilate and Atorvastatin Tablets

A simple, specific, accurate and precise high performance liquid chromatographic method was developed for the determination of Amlodipine and Atorvastatin in Amlodipine besylate and Atorvastatin tablet dosage forms. A Hypersil BDS C18 5μm (250x4.6mm I.D) with column temperature 30°C in isocratic mode, with mobile phase containing Acetonitrile: 10mM Ammonium acetate buffer pH 4.0 (50:50) was used. The flow rate was 1.0mL/min and effluent was monitored at 238nm.The retention time of Amlodipine was 2.5min and for Atorvastatin was 4.7min. The linearity for Amlodipine and Atorvastatin was in the range 0.02mg/mL to 0.12mg/mL. The proposed method is accurate, precise, specific and rapid estimation of Amlodipine and Atorvastatin in Amlodipine besilate and Atorvastatin tablets.