IFN-γ对白血病细胞株FBL-3细胞生物学行为的影响

Objective To investigate the effects of interferon-γ(IFN-γ) on proliferation,cell cycle,apoptosis and invasion of mouse leukemia cell line FBL-3.Methods The leukemia cellular proliferation capacity was determined with MTT assay,cell cycle distribution and apoptotic rate were measured with flow cytometry and the invasion capacity was assayed in a Transwell cell culture chamber.Result(1)Low dose of IFN-γ(5 ng/ml) had no significant effects on proliferation of FBL-3(P0.05).The survival rate of FBL-3 stayed up to about 80% after treated by IFN-γ(50~200 ng/ml) for 48 h while higher dose of IFN-γ(500 ng/ml) had significant suppressive effects(less than 50%)(P0.05);(2)The FBL-3 cell number at G0/G1 phase was increased and cell number at G2/M phase was relatively reduced after treatment,indicating that cells were arrested at G0/G1 phase by IFN-γ;(3)IFN-γ had promotive effects on apoptosis and suppressive effects on cell invasion capacity of FBL-3 cells in a dose-dependent manner(P0.05).Conclusion IFN-γ had definite effects against leukemia through suppressing proliferation,promoting apoptosis and decreasing cell invasion directly in a dose-dependent manner.