Inhibitory effects of IFN-α and lipopolysaccharide on proliferation of acute myeloma leukemia cell lines U937 and UL60 in vitro

Objective To investigate the effects of IFN-α in combination with lipopolysaccharide on the cellular proliferation,apoptosis and cell cycle of acute myelogenous leukemia(AML)cell lines U937 and HL60 in vitro.Methods The effects of IFN-α and lipopolysaccharide on AML cell lines U937 and HL60 proliferation were detected by MTT.Cells apoptotic rate and cell cycle were evaluated by using annexin-V FITC/PI apoptotic kit and flow cytometry,respectively.Results(1)We found that IFN-α or lipopolysaccharide alone could inhibit leukemic proliferation,increase cellular apoptotic rate and percentage of cells at G1 phase significantly,comparing with the control group(P0.05).(2)IFN-α in combination with lipopolysaccharide could inhibit leukemic proliferation,increase the cellular apoptotic rate and percentage of cells at G1 phase significantly,comparing with IFN-αor lipopolysaccharide alone and the control group,respectively(P0.05).Conclusion IFN-α in combination with lipopolysaccharide has synergistic effect on the proliferation inhibition and cellular apoptotic induction of AML cells.