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Anti-proliferative activity induced by interferon-lambda in human esophageal carcinoma cells and its mechanism

Feng Zhang

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Abstract

Objective To investigate the growth inhibition of IFN-lambda(IFN-λ) on 6 human esophageal carcinoma cells and analyze the mechanism.Methods The expression of IFN-λ receptors was examined with RT-PCR in 6 human esophageal carcinoma cells.The esophageal carcinoma cells were treated with IFN-λ,then the cell proliferation was evaluated with MTT assay.The kinetics of cell growth was examined with the trypan blue dye exclusion test.The distributions of cell cycle were analyzed with flow cytometer.Results Six esophageal carcinoma cells all expressed IFN-λ receptors.IFN-λ mediated the growth inhibition with the differential sensitivity.In TE11,YES5 and T.Tn cells,viable cell numbers after IFN-λ treatment reduced significantly.IFN-λ enhanced the G1 phase in TE11 cells and sub-G1 phase in YES5 and T.Tn cells.Conclusion IFN-λ can inhibit the growth of esophageal carcinoma cells in vitro through G1 phase arrest or apoptosis.

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What this paper is about

Objective To investigate the growth inhibition of IFN-lambda(IFN-λ) on 6 human esophageal carcinoma cells and analyze the mechanism.Methods The expression of IFN-λ receptors was examined with RT-PCR in 6 human esophageal carcinoma cells.The esophageal carcinoma cells were treated with IFN-λ,then the cell proliferation was evaluated with MTT assay.The kinetics of cell growth was examined with the trypan blue dye exclusion test.The distributions of cell cycle were analyzed with flow cytometer.Results Six esophageal carcinoma cells all expressed IFN-λ receptors.IFN-λ mediated the growth inhibition with the differential sensitivity.In TE11,YES5 and T.Tn cells,viable cell numbers after IFN-λ treatment reduced significantly.IFN-λ enhanced the G1 phase in TE11 cells and sub-G1 phase in YES5 and T.Tn cells.Conclusion IFN-λ can inhibit the growth of esophageal carcinoma cells in vitro through G1 phase arrest or apoptosis.

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Available abstract

Objective To investigate the growth inhibition of IFN-lambda(IFN-λ) on 6 human esophageal carcinoma cells and analyze the mechanism.Methods The expression of IFN-λ receptors was examined with RT-PCR in 6 human esophageal carcinoma cells.The esophageal carcinoma cells were treated with IFN-λ,then the cell proliferation was evaluated with MTT assay.The kinetics of cell growth was examined with the trypan blue dye exclusion test.The distributions of cell cycle were analyzed with flow cytometer.Results Six esophageal carcinoma cells all expressed IFN-λ receptors.IFN-λ mediated the growth inhibition with the differential sensitivity.In TE11,YES5 and T.Tn cells,viable cell numbers after IFN-λ treatment reduced significantly.IFN-λ enhanced the G1 phase in TE11 cells and sub-G1 phase in YES5 and T.Tn cells.Conclusion IFN-λ can inhibit the growth of esophageal carcinoma cells in vitro through G1 phase arrest or apoptosis.

Key concepts: Trypan blue, Cell cycle, Carcinoma, Cell growth, Apoptosis, Flow cytometry, Cancer research, Growth inhibition

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Anti-proliferative activity induced by interferon-lambda in human esophageal carcinoma cells and its mechanism — Research Paper | ScholarLens