2009•Chinese Veterinary ScienceRequires access
Preparation and biological characteristic of monoclonal antibodies against mink enteritis virus.
Jianke Wang, Shipeng Cheng, Xijun Yan, Yi Li, Luo Guo-liang, Chai XiuLi, Bin Luo, Wei Wu, Zhao Chuan-fang
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Abstract
Female BALB/c mice of four to six week-old female were immunized with the purified mink enteritis virus(MEV SMPV-11)strain,spleen cells were taken to fuse with SP2/0 myeloma cells and hybridoma cells were cloned by using indirect ELISA and limited dilution methods.8 hybridoma cell lines steadily secreting monoclonal antibodies(McAbs) against MEV were obtained and designated as D3,H5,F5,F4,A9,B7,H11 and H8,respectively.The ELISA titers of these McAbs ranged from 1∶6.4×103 to 1∶2.56×104.Specificity test showed that all the McAbs were specific to MEV,canine parvovirus and feline panleukopenia virus,but did not react with canine distemper virus,Aleutian disease virus and canine adenovirus.All the McAbs were identified to be IgG using Mouse Mab Isotyping Test Kit.