Establishment of hybridoma cell lines secreting monoclonal antibodies against pseudorabies virus

Balb/c mice were immunized with the purified PrV of Beijing isolate, mouse spleen cells were fused with myelomas SP2/0. Cultrue supernatants of hybridoms were screened by indirect ELISA, positive colonies were cloned three times by limited dilution. Two cell lines named 1H7 and 3B5 which could secrete McAbs stably were obtained. The isotypies of the two McAbs were IgG1, kappa light chain, the average number of chromosomes were 97, the ELISA titers of culture supernatants and ascities were 1∶1024, 1∶1024 and 1∶10 8、 10 7, respectively. The McAbs did not cross react with PRRSV、 HCV、 PPV, demonstrated good specifity, These results show that further application of the McAbs is possible.