Difference Spectroscopy Validated Method Development for Ciprofloxacin in Tablet by UV-Spectrophotometer

A simple, specific, precise and accurate Spectrophotometric method was developed for the determination of Ciprofloxacin by Spectrophotometric method in tablet dosage forms. Method was difference spectroscopy in which difference spectra were obtained by measuring the absorbance in acidic solution and basic solution. The drug shows absorption maxima and minima at wavelength 278 and 272 nm in basic and acidic solution respectively. The Method was validated according to ICH Guidelines. Spectrophotometric method linear response obtained was in the concentration range of 2–22 μg/ml with correlation coefficient 0.9993, recovery of the drug was found to be 99.40% and relative standard deviation was found to be less than 2% for precision studies. The newly developed methods can be used for routine analysis of Ciprofloxacin in tablet dosage forms.