2000•Journal of Clinical MicrobiologyOpen access

Evaluation of Random Amplified Polymorphic DNA Typing of Pseudomonas aeruginosa

M E Campbell, Eshwar Mahenthiralingam, David P. Speert

Abstract

A method for distinguishing among Pseudomonas aeruginosa strains using random amplified polymorphic DNA (RAPD) typing was evaluated for reproducibility and discriminatory power. A total of 200 isolates, blinded in triplicate, were evaluated by RAPD. All 600 samples were typeable; 197 of 200 isolates gave identical results on all three occasions, and 131 distinct RAPD types were identified.

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Key concepts: RAPD, Typing, Pseudomonas aeruginosa, Biology, DNA, Genetics, Pseudomonadaceae, Microbiology