[The study of combining high-risk human papillomavirus types checking and cytologic test in the screening of cervical lesions].

OBJECTIVE: To study the relationship between thinprep cytologic test and the types of human papilloma virus (HPV) infection in cervical precancerous lesion screening. METHODS: To perform high-risk HPV types test in 1375 samples. Choose 256 positive samples to take thinprep cytologic test (TCT) and directed biopsies under colposcopy. Adopting two-channels real time PCR to genotype and quantify eight high risk HPV DNA (high risk types: HPV 16, 18, 45, 31; intermediate risk types: HPV 33, 52, 58, 67). RESULTS: There are 256 positive samples in High risk HPV DNA test (18.62%). WNL rate for TCT is 16.41% (42/256), ASCUS and above rate for TCT is 83.59% (214/256). There is no statistically significant difference in the viral loads of HPV infection rate between the TCT negative patients and positive patients (P > 0.5). Positive correspondence rate for TCT and biopsy are 92.86% (39/42), 81.36% (48/59), 85.19% (23/27) and 9/10. CONCLUSION: High-risk HPV types checking combined with TCT and biopsy can raise positive rate significantly. It should be used as a reliable method for early diagnosis in cervical cancer and CIN screening.