2010•The Chinese Journal of Modern Applied PharmacyRequires access
Determination of Ephedrine Hydrochloride and Pseudoephedrine Hydrochloride Contents in Xiao'er Qingre Zhike Granules by HPLC
Shenggu Xie
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Abstract
OBJECTIVE To establish a HPLC method for the determination of ephedrine hydrochloride and pseudoephedrine hydrochloride contents in Xiao'er Qingre Zhike granules.METHODS Separation was performed on ZORBAX SB C18 column.0.1% phosphoric acid solution-acetonitrile(96∶4) was selected as the mobile phase,flow rate was set at 1.0 mL·min-1 and detection wavelength was 205 nm.RESULTS The linear range for ephedrine hydrochloride was 4.388-43.88 μg·mL-1(r=0.999 9),the average recovery of ephedrine hydrochloride was 99.7%,RSD=2.1%(n=6).The linear range for pseudoephedrine hydrochloride was 5.300-53.00 μg·mL-1(r=0.999 7),the average recovery of pseudoephedrine hydrochloride was 98.5%,RSD=2.8%(n=6).CONCLUTION The method is accurate and repeatable,and it can be applied to control the quality of this drug.