Content Determination of 3 Kinds of Alkaloids in Xiao'er Qingre Zhike Oral Liquid by CE

OBJECTIVE:To establish a method for the content determinnation of ephedrine hydrochloride,pseudoephedrine hydrochloride and methylephedrine hydrochloride in Xiao'er qingre zhike oral solution.METHODS:Capillary electrophoresis was adopted using berberine hydrochloride as internal standard.The separation was performed on a fused silica capillary column.40 mmol/L NaB 4 O 7 +15% ethanol(pH 9.96)was selected as the running buffer.The voltage applied was 12 kV.The sample was injected by gravity(10 s,15 cm).The detection wavelength was set at 190 nm.The column temperature was 25 ℃ and humidity was lower than 70%.RESULTS:The linear range of ephedrine hydrochloride,pseudoephedrine hydrochloride and methylephedrine hydrochloride were 2.4-30 μg/ml,1.2-15 μg/ml,0.308-3.85 μg/ml.The average recoveries were 96.3%,99.6% and 97.6%,respectively.RSDs were 1.30%,1.76% and 2.52%,respectively(n=6).CONCLUSION:The method is convenient,rapid,accurate and reliable for the quality control of Xiao'er qingre zhike oral solution.