Determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in xiaoerkechuanling granules by HPLC

Aim To develop a method for the determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Xiao er ke chuan ling Granules by HPLC.Methods Ephedrine hydrochloride and pseudoephedrine hydrochloride were determined by HPLC,Agilent-ODS-3(5 μm,4.6 mm×250 mm) column with Acetonitrile-0.1% H3PO4(5∶ 95) as the mobile phase was used.The flow rate was 1.0 ml·min-1 and the detective wavelength was 207 nm.The column temperature was 25℃.Results A good linear relationship at a range of 0.0306 ～0.306 μg for the ephedrine hydrochloride(r=0.999 9) was obtained.The average recovery was 99.52%,and RSD was 0.39%(n=9).A good linear relationship at a range of 0.0278～0.278 μg for pseudoephedrine hydrochloride(r=0.999 8) was also obtained.The average recovery was 99.49%,and RSD was 0.52%(n=9).Conclusion The method is simple,reproducible and accurate with a goood specificify,and can be used for the quality control of xiaoerkechuanling granules.