Quantification of Phenolic Compound Gallic Acid in Polyherbal Ranger Capsule by High Performance Chromatographic Method

A rapid, simple, sensitive, robust, economic and improved RP-HPLC method was developed for the estimation of Gallic Acid in Ranger Capsule, polyherbal formulation. The chromatographic conditions used for the separation was Phenomenex Luna C18 (2) (4.6 x 250mm, 5µ), rheodyne manual injector with capacity of 20µL and mobile phase comprised of water and acetonitrile (80: 20%v/v pH was maintained to 3 with Ortho Phosphoric acid). The flow rate was 1.0mL/min with detection at 272nm. The linearity was found to be in the range of 0.5-50µg/mL for Gallic acid with correlation coefficient of 0.9994. The proposed method is accurate with 100.36% - 100.97 % recovery and The Limit of Detection (LOD) and Limit of Quantification (LOQ) were found to be 0.0178µg/mL and 0.0539µg/mL respectively. The amount of Gallic acid in Polyherbal Capsule was found to be 0.50%.