Molecular cloning and tissue distribution of porcine Chemerin and its receptor.

The coding sequence of porcine Chemerin and its receptor gene were amplified based on the in silico sequence information in the study.Total RNA was extracted from all kinds of organizations and mRNA sequence of gene was amplified by RT-PCR using designed primers.The PCR products were ligated into the pMD-19T vector,and then transformed into competent cells of E.coli JM109.Porcine Chemerin gene cDNA sequence,was 558 bp in length,with an open reading frame of 492 bp encoding 163 amino acids.ChemerinR gene cDNA sequence,was 1 470 bp in length,with an open reading frame of 1 092 bp encoding a 363 amino acids.Homology analysis showed that porcine Chemerin and nucleic acid sequences,bovine,human and rat had the homology of 86.7%,79.1% and 73%,amino acid sequence had the homology of 83.1%,73.4% and 67.7%.Homology analysis showed that porcine ChemerinR and nucleic acid sequences,human,rat and mouse had the homology of 80.4 %,77.6% and 72.6%,amino acid sequence had the homology of 88.2%,80.7% and 79.3%.Semi-quantitative RT-PCR analysis showed that Chemerin mRNA ubiquitously expressed in various tissues tested,but ChemerinR preferentially expressed in the bladder,brain and muscle.