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Cloning and sequencing of heart fatty acid-binding protein gene in porcine

Xiaobo Chen, Guozhou Liao, Hua Qun-yi, Ge ChangRong

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Abstract

Heart fatty acid-binding protein gene isolated from porcine (Duroc×Landrace×Yorkshire) muscle tissue, were amplified by RT-PCR. The product of 0.124 kb fragment in length was obtained as expected. Then, the amplified fragment was cloned into the pDM18-T vector and identified by PCR and sequence. The results demonstrated that nucleotide homology with other published H-FABP genes was between 94%～100%, and deduced amino acid homology was between 100% respectively.

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What this paper is about

Heart fatty acid-binding protein gene isolated from porcine (Duroc×Landrace×Yorkshire) muscle tissue, were amplified by RT-PCR. The product of 0.124 kb fragment in length was obtained as expected. Then, the amplified fragment was cloned into the pDM18-T vector and identified by PCR and sequence. The results demonstrated that nucleotide homology with other published H-FABP genes was between 94%～100%, and deduced amino acid homology was between 100% respectively.

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Available abstract

Heart fatty acid-binding protein gene isolated from porcine (Duroc×Landrace×Yorkshire) muscle tissue, were amplified by RT-PCR. The product of 0.124 kb fragment in length was obtained as expected. Then, the amplified fragment was cloned into the pDM18-T vector and identified by PCR and sequence. The results demonstrated that nucleotide homology with other published H-FABP genes was between 94%～100%, and deduced amino acid homology was between 100% respectively.

Key concepts: Cloning (programming), Homology (biology), Gene, Biology, Nucleic acid sequence, Heart-type fatty acid binding protein, Molecular biology, Molecular cloning

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