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2010•World Applied Sciences JournalRequires access

The effects of pH levels and plant growth regulators on in vitro regeneration of almond (Prunus dulcis Mill.).

Akhtar Shekafandeh

Open publisher page 6 citations

Abstract

In this research, a factorial 4×3×4 with 4 replications in Completely Randomized design was used to study the effects of different auxin treatments (4 levels), pH(2 levels) and cultivars (3 levels) on regeneration and rooting of in vitro shoot tips excised from shoots cultured on media supplemented with 1.5-2 mg/l BA. The results showed both auxins (NAA and IBA) produced callus in the bases of the shoots. NAA treatment gave friable callus and IBA produced compact callus. Between two types of auxins used only shoots treated with IBA produced roots. PH levels had significant effect on rooting. The shoots of 'Shahrood 8' produced roots at pH 5.7 in the rate of 70%. And the shoots of 'Shahrood 10' generated roots at pH 4.8 at the rate of 60%.

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What this paper is about

In this research, a factorial 4×3×4 with 4 replications in Completely Randomized design was used to study the effects of different auxin treatments (4 levels), pH(2 levels) and cultivars (3 levels) on regeneration and rooting of in vitro shoot tips excised from shoots cultured on media supplemented with 1.5-2 mg/l BA. The results showed both auxins (NAA and IBA) produced callus in the bases of the shoots. NAA treatment gave friable callus and IBA produced compact callus. Between two types of auxins used only shoots treated with IBA produced roots. PH levels had significant effect on rooting. The shoots of 'Shahrood 8' produced roots at pH 5.7 in the rate of 70%. And the shoots of 'Shahrood 10' generated roots at pH 4.8 at the rate of 60%.

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Available abstract

In this research, a factorial 4×3×4 with 4 replications in Completely Randomized design was used to study the effects of different auxin treatments (4 levels), pH(2 levels) and cultivars (3 levels) on regeneration and rooting of in vitro shoot tips excised from shoots cultured on media supplemented with 1.5-2 mg/l BA. The results showed both auxins (NAA and IBA) produced callus in the bases of the shoots. NAA treatment gave friable callus and IBA produced compact callus. Between two types of auxins used only shoots treated with IBA produced roots. PH levels had significant effect on rooting. The shoots of 'Shahrood 8' produced roots at pH 5.7 in the rate of 70%. And the shoots of 'Shahrood 10' generated roots at pH 4.8 at the rate of 60%.

Key concepts: Shoot, Callus, Auxin, Prunus dulcis, Prunus, Horticulture, Botany, Chemistry

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The effects of pH levels and plant growth regulators on in vitro regeneration of almond (Prunus dulcis Mill.). — Research Paper | ScholarLens