Effect of different concentrations of auxins on in vitro rooting of olive cultivar 'Moraiolo'.
Ansar Ali, Touqeer Ahmad, Naser Abbasi, I. A. Hafiz
Abstract
Ansar Ali, Touqeer Ahmad, Naser Abbasi, I. A. Hafiz
Abstract
The inability to induce adventitious roots is often a limiting factor in conventional cuttings and tissue culture. In this study, several criteria were taken into consideration in determining the best rooting treatment in olive cultivar Moraiolo. Among the indolebutyric acid (IBA) and Naphthaleneacetic acid (NAA) hormones, tested for maximum percentage of rooted shoots, root number, root length and quality of roots, IBA at 1.5 mg l -1 concentration proved to be the best one for rooting of Moraiolo cultivar of olive producing maximum root initiation in 86.67% shoots, 5.03 number of roots per rooted explant and 4.95 cm root length. The roots produced on IBA were longer with better quality shoots whereas NAA produced poor response with necrotic leaves and leaf abscission.
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The inability to induce adventitious roots is often a limiting factor in conventional cuttings and tissue culture. In this study, several criteria were taken into consideration in determining the best rooting treatment in olive cultivar Moraiolo. Among the indolebutyric acid (IBA) and Naphthaleneacetic acid (NAA) hormones, tested for maximum percentage of rooted shoots, root number, root length and quality of roots, IBA at 1.5 mg l -1 concentration proved to be the best one for rooting of Moraiolo cultivar of olive producing maximum root initiation in 86.67% shoots, 5.03 number of roots per rooted explant and 4.95 cm root length. The roots produced on IBA were longer with better quality shoots whereas NAA produced poor response with necrotic leaves and leaf abscission.
Key concepts: Auxin, Shoot, Cultivar, Cutting, Explant culture, Abscission, Biology, Horticulture