Effect of some growth regulators on "in vitro" culture of two Vitis vinifera L. cultivars.

Shoot apical meristems were used for regeneration 2 grape cultivars -Soltanin and Sahebi. Meristems were taken from 10 cm length shoots. The culture media used this study was MS (Murashige & Skoog, 1962), with hormonal supplements consisting of: A (1 mgL -1 BA), B (1.5 mgL -1 BA), C (1 mgL -1 IBA+ 1.5 mgL -1 BA) and D (1 mgL -1 TDZ). Results indicated that B and C variants of culture media produced the highest average number of shoots per cultured apex (3.8-5.4). Consequently, the shoots were twice subcultured on a medium variant supplemented with 1 mg/L TDZ+1.5 mg/L GA. The in vitro derived shoots were pretreated with 1 mgL -1 IBA, and then directly potted, which caused significant enhancement root number per shoot. The good rooting achieved under in vivo conditions resulted the reduction of the time spent for vitroplant acclimatization compared with in vitro rooting and, therefore, the time needed for plantlet regeneration was shortened. There was no significant difference between the two cultivars regarding the measured traits.