Factors Affecting the Efficiency of Agrobacterium-Mediated Gene Transformation in Indica Rice
Arega Gashaw
Abstract
Arega Gashaw
Abstract
Agrobacterium-mediated gene transformation has been commonly used to transfer desirable foreign gene from any source to rice genome. However, indica rice was considered recalcitrant to Agrobacterium- mediated gene transformation. Through optimization of relevant transformation techniques, several indica rice varieties were successfully transformed. This mini-review compiles the success stories and future research focus on Agrobacterium-mediated gene transformation technique in indica rice. Reasons that hinder the efficiency of Agrobacterium-mediated gene transformation, and factors that improve its efficiency are reviewed.
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Agrobacterium-mediated gene transformation has been commonly used to transfer desirable foreign gene from any source to rice genome. However, indica rice was considered recalcitrant to Agrobacterium- mediated gene transformation. Through optimization of relevant transformation techniques, several indica rice varieties were successfully transformed. This mini-review compiles the success stories and future research focus on Agrobacterium-mediated gene transformation technique in indica rice. Reasons that hinder the efficiency of Agrobacterium-mediated gene transformation, and factors that improve its efficiency are reviewed.
Key concepts: Agrobacterium, Transformation (genetics), Biology, Gene, Gene transfer, Biotechnology, Transformation efficiency, Genetics