Development of Analytical Method of Melamine Residues in Chicken Serum by HPLC

Chicken serum in this experiment was as the research object and the different washing liquid and the eluate concentration on the extraction efficiency of melamine were inspected.A simple high-performance liquid chromatography (HPLC) with NH_2 column and 95%acetonitrile eluate to determine the melamine in chicken serum was described.Target analytes of samples were cleaned up and concentrated by HyperSep Retain-CX column solid-phase extraction(SPE).A perfect separation for melamine was achieved and retention time was 14-15 min at 214 nm.The standard curve was linear in the concentration range from 0.05μg/ml to 5.0μg/ml,with correlation coefficient of 0.999 9.Recoveries of melamine at fortified levels of 0.1μg/ml,1.0μg/ml and 5.0μg/ml ranged from 78.05%to 88.69%,with relative standard deviation of 1.15%-8.33%.The quantification limit was 0.1μg/ml.