2012•African Journal of Microbiology ResearchOpen access

Expression of S1 glycoprotein gene of infectious bronchitis virus (IBV) in Escherichia coli

Sepideh Ghani, Seyed Davood Hosseni, Mohammad Reza Zolfaghari, Shahin Masoodi

Abstract

The objective of this work is to provide S1 protein as a recombinant antigen in order to be used in diagnostic kits. Various strains of infectious bronchitis virus (IBV) were detected by the S1 glycoprotein gene. A prevalent strain 4/91 was frequently isolated in material from Iran. In this study we extracted RNA from vaccine strain 4/91 of IBV and amplified the S1 gene using RT-PCR and then cloned the S1 glycoprotein gene of infectious bronchitis virus into a cloning and expression vector. The result confirmed that a fragment of 900 bp in size was expressed and amplified. The results also showed that a protein about 33 KDa was expressed. Additionally, western blot assay confirmed the expression of S1 protein. Key words: infectious bronchitis virus, S1 glycoprotein, cloning, E. coli.

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Key concepts: Infectious bronchitis virus, Virology, Avian infectious bronchitis virus, Recombinant DNA, Cloning (programming), Gene, Glycoprotein, Biology