Components of a Microinjection System

INTRODUCTION Direct-pressure microinjection with a micropipette is an essential tool for introducing a variety of impermeant substances into the cytoplasm or nucleus of plant and animal cells. Microinjection remains the most direct method to gain insight into the function and dynamics of intracellular components, to produce transgenic animals, or to overcome male infertility. This article describes the basic components of a microinjection system.