2011•International Journal of Animal and Veterinary AdvancesRequires access
Correlation of Chemiluminescence and Electrochemiluminescence Methods for Determination of Serum Free Triiodothyronine Level in Cow
Behrad Eshratkhah, Mehrdad Mostafaei Bigham, Alireza Mirzaei, Rahim Beheshti
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Abstract
The aim of this study was to determine serum free-triiodothyronine (fT3) level and its correlation between the CLIA and ECLIA methods in cow. Blood samples were collected from the jugular vein of 25 clinically healthy animals aged from 2 to 4 years. This study was performed in August. The separated sera were analyzed to measurement of fT3 concentration using the CLIA and ECLIA methods. Our results indicate that there was no a significant difference between two method concerning the fT3 concentration in cow, but its mean was higher in CLIA method. The regression analysis revealed a significant correlation between two methods: (p = 0.022, r = 0.460 for fT3). It was concluded that the CLIA and ECLIA methods can be used as an alternative to the previous methods for assaying the fT3 concentration in veterinary diagnostic laboratories and exhibited an acceptable of sensivity and precision for the serum fT 3 determination in cow.