Separation and purification of chlorogenic acid from Lonicera japonica Thunb. leaves exact with macroporous resins

Chlorogenic aicd is a main bioactive constituent in Lonicera japonica Thunb. leaves. In this study, a simple method for separation and purification of chlorogenic acid from L. japonicaleaves was established with macroporous resins. The performance and adsorption characteristics of four macroporous resins had been evaluated and D-101 was affirmed to be the suitable resin for the separation of chlorogenic acid. Dynamic adsorption and desorption tests performed in a column packed with D-101 resin were followed to optimize the process parameters. The final results showed that the highest purity of the chlorogenic acid product was 50.72% with a recovery yield of 54.44% when the parameters were as follows: a sample concentration in solution of 1.01 mg/mL chlorogenic acid, a pH value of 4, a flow rate of 2 bed volume (BV)/h, a sample volume of 5 BV and an elution solvent of 20% (v/v) ethanol.   Key words: Macroporous resins, Lonicera japonica Thunb. Leaves, chlorogenic acid, adsorption, desorption ratios.