Experimental study on anti-colorectal cancer efficacy of extracts of TCM Compound AK combined with cisplatin

Objective:To investigate the toxicity-reducing and synergistic effects of the extracts of AK prescription for chemotherapy.Methods:Models of colorectal CT-26 tumor bearing mice were established and randomly divided into six groups,including group A(control group),group B(CDDP group),group C(AK group) and combined treatment groups D,E and F.The medication was begun after 24 hours since inoculation,once a day for 12 days.Mice were sacrificed on day 14th.The tumor inhibitory rate,thymus index,spleen index,liver and renal function and the blood cell in the peripheral blood were measured.The subtypes of T lymphocytes in the peripheral blood were detected by flow cytometry.Optical microscopy was employed to observe the structure of tumor and renal.Results:①The tumor weight in groups D,E and F was lighter than that in group A(P0.05).Groups B,C,D,E and F could inhibit the tumor growth and the tumor inhibition rate was 55.92%,28.11%,61.05%,71.11%,69.72%,respectively.②Compared with group A,in group B the count of WBC and PLT was decreased obviously and the level of blood urea nitrogen(BUN) was increased(P0.01).Compared with group B,in groups D,E and F the count of WBC and PLT was increased and the level of BUN was decreased(P0.01).③Compared with group A,in group B the level of CD3+,CD4+,CD4+/CD+8 was lower and the level of CD+8 was higher(P0.05).The level of CD3+,CD4+,CD4+/CD+8 in groups D,E and F,was higher than that in group B,but CD+8 value was decreased(P0.01).④Compared with group A,thymus index and spleen index were decreased in group B(P0.05).Groups D,E and F had different levels ofincrease in thymus index and spleen index compared with group B(P0.05).Conclusion:The extracts of prescription of TCM AK can display the toxicity-reducing and synergistic effects for chemotherapy.