Immune Function Indicators and Effect of Cyclophosphamide on H22 Solid Tumor-bearing Mice

Objective Standardized determination of immune function indicators and effect of cyclophosphamide on the H22 solid tumors in mice.Method H22 cells were implanted into the left axilla of 46 BALB/c mice.Tumors growth was observed 1 days later,The mice were randomly divided into tumor-bearing mice+cyclophosphamide group,tumor-bearing mice group,control mice+cyclophosphamide group,control mice group.Cyclophosphamide group intraperitoneal injection of cyclophosphamide 50mg/kg,2 times a week.Observe the general clinical manifestations,tumorigenicity,body weight,food intake,tumor volume,tumor weight,immune organ weight,blood values,blood biochemistry values,cytokines,lymphocyte transformation,NK cell activity,lymphocyte subsets and histopathology.Result Compared with normal mice,H22 tumor-bearing mice reduced food consumption,RBC,HGB,HCT significantly decreased(P0.01),WBC,PLT,RDW significantly increased(P0.01),GOT,GPT significantlyincreased(P0.01),CD4-CD8-significantly decreased(P0.01).The average food intake reduces the tumor-bearing mice given cyclophosphamide,D9 to D14 body weight was significantly lower(P0.05),D5 to D14 tumor volume was significantly lower(P0.01),tumor weight,spleen weight,thymus weight was significantly sexual lowering(P0.01),the WBC,MCV significantly with lowering(P0.01),HGB,the MCH,MCHC,the PLT significantly with increased(P0.05),the GOT,GPT significantly(P 0.01) lower of IL-2 significantly with reducing(P0.01),IFN-γ significantly increased(P0.01),CD4+CD8-(%),CD4+CD8+(%),CD4-CD8+(%),CD3+(%) lymphocytes significantly with increased(P0.01),CD4-CD8-(%) lymphocytes was significantly lower(P0.01), spleen lymphocytes transformed absorbance difference significantly lower(P0.05),NK cell activity significantly reduce(P0.05).Conclusion Blood parameters and immune function indicators were measured in H22 tumor-bearing mice.We provide the basic data for biological response modifiers anticancer drug screening.